The amount of bio-media needed depends on factors such as water quality, population density cultivation, water circulation, etc.
The starting guide is as follows:
For larvae in hatchery tanks: 1 pound/1000 gal
Fish or shrimp tanks: 2 pounds/ 1000 gal
Ponds: 10 pounds / per acre
It is recommended that the bio-media is installed about 3-7 meters from the aeration systems (if in use) symmetrically distributed in areas where the water flows carrying the bacteria to different points of the pond or tank.
The Bio-media will last for two years, if used correctly.
The inoculation process can be accelerated, by soaking the bio-media in water saturated with bacteria before being installed.